SNIPe

Selecting Novel Informative Primer-sets for e-DNA

Choose or upload a dataset

Discover primer pair sets

Use primer dataset from Tournayre et al.

You must upload a CSV which follows the tool's formatting requirements.
SNIPe can be used with any dataset which uses this format, including lists generated by in-silico analyses.

Primers

Taxa

Note: All processing is done on your local computer; datasets are not sent to a remote server.

SNIPe is a primer pair selection tool for environmental DNA / metabarcoding studies, and an exploration tool for Tournayre et al.'s freshwater diversity metabarcoding dataset, which can be used to find informative subsets of validated primer pairs that maximize detection of taxa of interest.

If you use SNIPe in your work, please cite:

Enhancing metabarcoding of freshwater biotic communities: a new online tool for primer selection and exploring data from 14 primer pairs
Orianne Tournayre, Haolun Tian, David R. Lougheed, Matthew J.S. Windle, Sheldon Lambert, Jennipher Carter, Zhengxin Sun, Jeff Ridal, Yuxiang Wang, Brian F. Cumming, Shelley E. Arnott, Stephen C. Lougheed. (2024).
Environmental DNA 6, e590; DOI: 10.1002/edn3.590

This web application is designed to complement Tournayre et al. (2024). The data presented here were generated by Orianne Tournayre and other members of the Lougheed Lab. The web application component is © David Lougheed 2023-2024; the source code is available on Github under the terms of the GPL v3 license; see the notice.

Version 1.1.2 ⸱ Please send any feedback or bug reports to david.lougheed@gmail.com or file an issue on GitHub.